Characteristics of Metabolites by Seed-Specific Inhibition of <i>FAD2</i> in <i>Brassica napus</i> L

Fatty acid desaturase-2 (FAD2) is a key enzyme in the production of polyunsaturated fatty acids plants. RNAi technology can reduce expression FAD2 genes Brassica napus seeds and acquire transgenic B. plants with high oleic content, but effect seed-specific inhibition on seed metabolites not clear. Here we use widely targeted metabolomics to investigate normal-oleic-acid rapeseed (OA) high-oleic-acid (HOA) seeds, resulting total 726 being detected. Among them, 24 differential were significantly downregulated 88 upregulated HOA rapeseed. In further lipid profile experiments, more lipids accurately quantified. The contents glycolipids phospholipids that contain C18:1 increased C18:2 decreased because was inhibited. changes related pathways also consistent metabolites. insertion site ihpRNA plant vector reconfirmed through genomewide resequencing, event did change sequence genes. There no significant difference germination rate potential between OA affect other stages growth. This work provides theoretical practical guidance for subsequent molecular breeding napus.